FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l02n01_nutrinetat_vegc27_sakata_r2y_ln.35100000089dd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l02n01_nutrinetat_vegc27_sakata_r2y_ln.35100000089dd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24878454
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCC3416901.3734374330494974TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC383950.042.24819645
ACGTCTG395050.041.873515
GCACACG398150.041.56441511
CACACGT404900.040.9159412
AGCACAC406600.040.7116810
CGTATGC398500.040.6869744
CTCGTAT399500.040.618942
TCGTATG407150.039.93304443
ACACGTC415500.039.85587713
CACGTCT415200.039.85217714
GAGCACA421400.039.393939
TCGGAAG420650.039.2159653
CGTCTGA428050.038.72937816
GATCGGA426550.038.612711
TCTCGTA422150.038.4608541
ATCGGAA433650.038.0274352
CAGTCAC432850.037.73428327
CTGAACT440650.037.46507619
AATCTCG433250.037.43913339
CCACTCA436150.037.3605233
Produced by FastQC (version 0.11.2)