FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l02n01_nutrinetat_vegc25_oy-0_r2y_ln.35100000089d5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l02n01_nutrinetat_vegc25_oy-0_r2y_ln.35100000089d5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136809
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC25640.11999200677271576TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG4900.029.83570315
GCACACG5250.027.84665511
AGCACAC5850.025.75953110
CACACGT5850.024.9905912
CTCGTAT5550.024.72041942
TATCTCG5400.024.57407839
TCGTATG5950.023.43655243
GTATGCC5800.023.32276545
CACGTCT6450.022.66588214
ACACGTC6600.021.80996913
CGTATGC6100.021.754144
AGTCACT6600.020.45402528
GAGCACA8000.019.6800889
CACTAGC6700.019.1399631
ACTAGCT6800.018.85760532
TCGGAAG7950.018.7107413
TAGCTTA7150.018.55944434
GCTTATC7650.018.52243636
CGTCTGA8150.018.4899716
TCTCGTA7550.018.17196541
Produced by FastQC (version 0.11.2)