FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l02n01_nutrinetat_vegc23_n13_r2y_ln.35100000089ce3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l02n01_nutrinetat_vegc23_n13_r2y_ln.35100000089ce3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22831221
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCA11100.014.8254451
GCCGGAT33750.013.9598371
GCGCGCG9100.013.8705132
GCGGGTT46100.012.9584421
GGGCGCT4550.012.88161
GGGGGAT39400.012.4157031
GCCGGTT39950.012.0754921
GGGCGCC2251.0181957E-612.0228261
GCGGGCT11350.011.7182821
GGCAGGT34650.011.5153691
CGGGCCT13300.011.3561471
GGGGATA46800.011.2697922
CGCGGGG16050.011.07691525
TCGGGCG10850.010.9859347
GTCGGAT61800.010.9795831
CCGGGTT57050.010.9454081
CGCGCGT11450.010.8264233
CGGGCGC10400.010.81248815
CCCGGGT34550.010.63524151
GCCGGGT28750.010.5853151
Produced by FastQC (version 0.11.2)