FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l02n01_nutrinetat_vegc21_mt-0_r2y_ln.35100000089c86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l02n01_nutrinetat_vegc21_mt-0_r2y_ln.35100000089c86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49013888
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG868700.17723548068661682TruSeq Adapter, Index 22 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG637710.13010802162848212No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA592710.12092695033701469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA118900.034.9368935
CGTACGT124450.033.34272834
CCGTACG136200.030.81323233
TACGTAA140500.029.69386936
ACGTCTG147250.029.35602615
GCACACG157350.027.60035911
TCGTATG154500.027.02250745
ACCGTAC156600.026.83539232
ACGTAAT165150.025.7656937
CACACGT169550.025.60110712
AGCACAC174950.024.96516810
CTCGTAT169800.024.8349244
ACACGTC176850.024.49347513
CACGTCT195650.022.2548514
GAGCACA202450.021.8739459
TCTCGTA210150.020.36613543
CGTCTGA217500.020.10185816
TCGGAAG217150.020.0405243
CAGTCAC215800.019.9286127
ACTCCAG235900.018.58891923
Produced by FastQC (version 0.11.2)