FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l02n01_nutrinetat_vegc19_mr-0_r2y_ln.35100000089c6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l02n01_nutrinetat_vegc19_mr-0_r2y_ln.35100000089c6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40379015
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG2411070.5971096620360848TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG301750.038.48297533
ACGTCTG310300.038.13247315
GCACACG318550.037.18725611
GTCACGT314600.036.97269429
AGTCACG317400.036.76010528
TCGTATG315200.036.61705445
CACACGT323950.036.52571512
CTCGTAT318100.036.36313244
AGCACAC328350.036.17325210
ACACGTC329550.035.83679213
CACGTCT346400.034.1909714
GTTTCGG342350.034.00422734
GAGCACA355750.033.513629
CACGTTT352850.033.13266431
CGTCTGA358050.033.07220516
TCGGAAG361050.032.6879463
CAGTCAC365000.032.20754227
TCTCGTA362150.032.13263743
ACGTTTC366300.031.95551532
TTTCGGA369250.031.52087635
Produced by FastQC (version 0.11.2)