FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l01n01_nutrinetat_vegc9_ct-1_r2y_ln.35100000089f33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l01n01_nutrinetat_vegc9_ct-1_r2y_ln.35100000089f33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8590431
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCC156350.18200483770837575TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG24500.030.75757615
GTATGCC25100.028.97338745
GCACACG27800.027.34940311
CGTATGC26950.026.95983344
CACACGT29900.025.65423812
ACACGTC29300.025.56539713
CTCGTAT28750.025.03718842
TCGTATG30950.023.83892343
CACGTCT31850.023.7304414
AGCACAC32400.023.2581810
GATCGGA35250.021.102511
TCGGAAG36000.020.8845883
TCTCGTA35350.020.42769441
CGTCTGA37200.020.31747616
GAGCACA37950.020.2717069
CAGTCAC37500.019.68376427
ATCGGAA38050.019.3457322
CTGAACT42350.018.11317619
TCCAGTC41250.017.89620625
ACTCCAG43400.017.8392823
Produced by FastQC (version 0.11.2)