FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l01n01_nutrinetat_vegc7_col-0_r2y_ln.35100000089eae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l01n01_nutrinetat_vegc7_col-0_r2y_ln.35100000089eae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24526488
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1423010.5801931364979772TruSeq Adapter, Index 10 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG358770.14627858664477358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC170100.039.89233445
ACGTCTG182850.038.15004715
CGTATGC177600.038.09984244
GCACACG184350.037.68100411
TATCTCG183800.036.8039839
CTCGTAT186050.036.40569742
TCGTATG187050.036.24714743
CACACGT192050.036.09995312
ACACGTC195400.035.7573413
AGCACAC194950.035.7014110
AGTCACT194000.035.14208228
TAGCTTA196400.034.8314934
ACTAGCT196850.034.66221232
CACGTCT201950.034.4973414
CACTAGC197650.034.40907731
GAGCACA211900.033.004879
CGTCTGA213250.032.8275816
TCACTAG207650.032.7874530
TCTCGTA207200.032.76697541
GCTTATC208200.032.71764436
Produced by FastQC (version 0.11.2)