FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l01n01_nutrinetat_vegc5_bur-0_r2y_ln.35100000089e27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l01n01_nutrinetat_vegc5_bur-0_r2y_ln.35100000089e27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34931361
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG359610.10294760630712328TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG78650.023.42480311
ACGTCTG78700.023.2384215
AGTCACG78950.022.05875428
ACGAGTG82300.021.10270332
TATCTCG83600.020.85396841
CACACGT90850.020.2791512
AGCACAC91600.020.11311110
CACGAGT87500.019.6950431
TCGTATG89700.019.4765845
GTCACGA90000.019.17473829
ACACGTC96300.019.08475513
CGAGTGG92200.018.61642333
CTCGTAT95750.018.23113644
CACGTCT102700.018.22399914
GGGGGAT56050.017.129411
GAGCACA111650.017.0049179
TCACGAG112600.015.66586230
CGTCTGA120550.015.48822516
GGATATC120600.014.56790238
TCGGAAG128800.014.3996753
Produced by FastQC (version 0.11.2)