FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l01n01_nutrinetat_vegc3_bi-1_r2y_ln.35100000089dbb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l01n01_nutrinetat_vegc3_bi-1_r2y_ln.35100000089dbb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35907171
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG1376170.38325770637848355TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA177150.038.4524135
CCGTACG178750.038.38445733
CGTACGT180300.037.7797734
ACGTCTG192800.036.4263315
TACGTAA188700.036.05061736
GCACACG197500.035.41155211
ACCGTAC200800.034.2046632
CACACGT207200.033.7863412
ACGTAAT205100.033.39830837
AGCACAC209950.033.29022210
TCGTATG206450.033.26204745
CTCGTAT206650.033.22298444
ACACGTC213100.033.0092613
CACGTCT220900.031.89461714
GAGCACA236700.029.8226389
TCGGAAG239100.029.1928633
TCTCGTA237300.029.1404143
CGTCTGA243250.028.95474416
CAGTCAC246700.028.0859927
GATCGGA250650.027.917841
Produced by FastQC (version 0.11.2)