FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l01n01_nutrinetat_vegc1_akita_r2y_ln.35100000089d42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l01n01_nutrinetat_vegc1_akita_r2y_ln.35100000089d42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31604257
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG921110.29145124341951784TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG131650.033.74720411
CGTTTCG129300.033.24830633
ACGTCTG135900.032.6253615
GTCACGT137000.031.56452629
AGTCACG141250.030.88675728
CACACGT145350.030.65921212
TCGTATG142600.029.83834845
AGCACAC149200.029.7776110
CTCGTAT146750.029.15550444
ACACGTC153300.029.12790313
CACGTCT157400.028.29771414
GTTTCGG158050.027.2568834
GAGCACA166650.026.956559
CACGTTT169750.025.56551631
TCGGAAG176500.025.3647023
CGTCTGA177550.025.09874216
CAGTCAC176400.025.01646227
TCTCGTA176950.024.34480943
ACGTTTC180150.024.21439432
TTTCGGA178750.023.937835
Produced by FastQC (version 0.11.2)