FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l01n01_nutrinetat_vegc17_mh-1_r2y_ln.3510000008a171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l01n01_nutrinetat_vegc17_mh-1_r2y_ln.3510000008a171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19668734
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCC3312951.6843737883688905TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG392900.042.50479515
GTATGCC375000.042.24691845
GCACACG396400.042.02789311
CGTATGC380950.041.5588344
ACACGTC402200.041.54475813
AGCACAC401450.041.46558810
CACACGT402200.041.39385212
TATCTCG383450.041.31543739
CTCGTAT385450.041.02112242
TCGTATG389350.040.6737843
CACGTCT410950.040.6547114
GAGCACA413750.040.2818269
TCGGAAG414600.039.762213
CGTCTGA421350.039.68287716
GATCGGA419250.039.4105641
CATTTTA407050.039.32180834
TCTCGTA404500.039.2043341
CAGTCAC416250.038.97802427
CTGAACT428200.038.94596519
ATCGGAA426750.038.6622772
Produced by FastQC (version 0.11.2)