FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l01n01_nutrinetat_vegc15_kn-0_r2y_ln.3510000008a0c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l01n01_nutrinetat_vegc15_kn-0_r2y_ln.3510000008a0c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19045715
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCC3940832.0691425866658197TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC441200.042.75753845
ACGTCTG462000.042.68200715
GCACACG464900.042.3723211
CGTATGC446750.042.21550844
CACACGT467650.042.0221412
AGCACAC471000.041.86175510
ACACGTC474150.041.63109213
CTCGTAT453950.041.49143242
TCGTATG457100.041.26947443
CACGTCT479250.041.13643614
TCGGAAG477050.040.8610573
GAGCACA484850.040.7494669
CGTCTGA486000.040.61590616
GATCGGA487250.040.1748661
TCTCGTA470550.040.08144441
ATCGGAA488450.039.9585082
CAGTCAC482850.039.6802527
ATGGCAT476100.039.6194535
CTGAACT496850.039.6176419
AGAGCAC500700.039.477498
Produced by FastQC (version 0.11.2)