FastQCFastQC Report
Sun 16 Apr 2017
C99B1ACXX_l01n01_nutrinetat_vegc13_ge-0_r2y_ln.3510000008a022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l01n01_nutrinetat_vegc13_ge-0_r2y_ln.3510000008a022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53670014
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG7300651.3602847206263071TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC839450.042.0542433
ACGTCTG870800.041.9234915
ACGTGGC851300.041.51831432
GCACACG878300.041.36584511
GCCTTAT860300.041.1446637
CACGTGG862800.041.09736631
GTCACGT860600.041.07534829
CACACGT890600.040.7642312
TATCTCG869950.040.7011941
TCACGTG873100.040.55428730
AGTCACG881350.040.5080928
CTCGTAT873750.040.4406244
AGCACAC899700.040.39442410
ACACGTC905900.040.31910313
TCGTATG877000.040.30602345
GTGGCCT877000.040.24035634
GGCCTTA887150.039.97293536
CACGTCT916750.039.8394614
GAGCACA932700.039.0737579
TCGGAAG930450.038.9026643
Produced by FastQC (version 0.11.2)