FastQCFastQC Report
Sun 16 Apr 2017
C99B1ACXX_l01n01_nutrinetat_vegc11_edi-0_r2y_ln.35100000089fb9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l01n01_nutrinetat_vegc11_edi-0_r2y_ln.35100000089fb9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23990420
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCC1728570.7205251096062512TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCGA210250.040.46608434
GTATGCC212150.040.3651445
ACGTCTG221450.040.03351615
CCAGGCG218700.039.07785433
GCACACG227750.039.04488411
AGGCGAT218800.038.88569335
CGTATGC220050.038.8774744
CACACGT231200.038.29684412
GGCGATC221000.038.2839536
CTCGTAT225050.037.97373242
AGCACAC235750.037.7008410
ACACGTC235900.037.6291913
TCGTATG228100.037.485743
CACGTCT238100.037.281514
ACCAGGC233000.036.84510832
GCGATCT233900.036.32640537
GAGCACA252650.035.250239
CGTCTGA254400.034.8925716
TCTCGTA247900.034.52898441
TCGGAAG254650.034.5242463
Produced by FastQC (version 0.11.2)