FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l08n02_182.3520000006b22b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l08n02_182.3520000006b22b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9317314
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG56400.024.5223351
CTATACT73500.022.0410924
CCTATAC61100.019.905123
TATACTG55800.019.6668365
TATAGGG54900.019.6458532
CCCTATA58250.017.292482
TATACTA92500.017.1538455
TAGACTG29050.017.0076355
ATAGGGA58400.015.7817433
GTATATA119150.015.6365151
TATACCG29250.015.2671775
TAGGGAT64600.015.1495874
TACACCG30350.014.8703675
TAATACT66500.014.7167414
GTATTAT59550.014.6853391
GTATAAG40250.014.6421131
TATACAG52800.014.5759825
TTATACT66900.014.4157094
ACTGTGC42400.014.3395518
ATACTGT57750.014.3121556