FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l08n02_176.3520000006b1d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l08n02_176.3520000006b1d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18112949
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG110400.018.9337335
GTATTGG156550.016.3327541
CTATACT113700.015.45966154
ACTGTGC77500.015.1998448
TTATACT136350.015.1562864
TAGACAG61050.015.0962385
GTATATA243500.015.0092021
TACACAG79800.015.0020415
TATACAG99900.014.9319625
GTATAGG75000.014.8914431
TAATACT132500.014.8795894
TACACCG58500.014.4549585
CTACACT83000.014.4238484
GTATTAT127000.014.2951871
TACACTG83850.014.22077755
TAGACTG59800.014.1407215
GTATAGA95650.014.06150151
GTATAAG76600.013.8979091
ATTATAC117850.013.8671623
GTATTAG85400.013.7457761
Produced by FastQC (version 0.11.2)