FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l08n02_174.3520000006b0da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l08n02_174.3520000006b0da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15620278
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG105600.019.6137265
CTATACT104100.017.249864
TATACAG97550.017.2390585
GTATAGG71350.016.5183891
TAATACT134600.016.4822444
TTATACT140050.016.3835684
CCTATAC69100.016.2247663
TAGACTG51700.015.9880785
TATACCG53950.015.8496095
GTATTGG129050.015.5404791
GTATAAT122750.015.5250441
ATAATAC122450.015.4795593
TCTATAC83300.015.2268733
GTATTAT121150.015.1416731
ATTATAC120300.015.0848923
GTATTAG82050.015.0013791
ATACTGT108600.014.6961146
GTATATA238150.014.3878091
GTATAAG73800.014.1669481
GTAATAC78050.013.4512863
Produced by FastQC (version 0.11.2)