FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l08n02_172.3520000006b07d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l08n02_172.3520000006b07d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20909489
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG122250.021.498511
CTATACT159400.019.8490314
CCTATAC130800.019.4675123
TATAGGG115800.017.3969042
TATACTG125900.017.2817175
TATACTA213950.015.853795
CCCTATA121900.015.6688472
TATACAG119000.015.5691475
GTATTGG129300.015.0333881
CTACACT91500.014.9528784
GTATATA278350.014.88872051
GTATTAT139300.014.5682991
TCTATAC101200.014.3646263
TAGACTG71150.014.288485
GTGTAGG84600.014.2690631
TAGACAG68500.014.1477295
TTATACT154400.013.815054
ACTGTGC96250.013.7192048
TACACAG88650.013.5577925
GTATAAG95750.013.550521
Produced by FastQC (version 0.11.2)