FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l08n02_168.3520000006b010.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l08n02_168.3520000006b010.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37554210
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG239800.021.5433141
CTATACT285150.020.5753964
TATACTG217900.019.686995
CCTATAC249600.019.6041153
TATAGGG223750.017.6462862
TAATACT285000.017.3691064
TTATACT289050.017.1586114
TATACTA384850.016.8496135
TATACCG122650.016.7713935
GTATTAT265300.016.1943441
GTATTAG175350.015.9097931
CCCTATA229600.015.7894852
GTATATA511350.015.4191471
ATTATAC251800.015.395333
ATAATAC256950.015.3825853
GTATTGG219550.015.3694061
GTATAAT267000.015.2368341
TATACAG205950.015.2240755
TAGACTG125550.015.0218225
GTATTAC165700.014.9433311
Produced by FastQC (version 0.11.2)