FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l08n02_164.3520000006afc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l08n02_164.3520000006afc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8332946
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT66250.022.3015234
CCTATAC54650.021.6455783
GTATAGG48050.019.4854351
TTATACT70350.018.8408344
TATACTG51700.018.3779435
TAATACT69950.017.997754
GTATTAG38800.017.5162681
CCCTATA51250.017.2441442
TATACTA96150.017.0461865
ATAATAC64800.016.5688923
TATACCG26450.016.5241685
GTATAGA44900.016.3009031
GTATAAG39300.016.2050191
GTATAAT64700.015.7197921
GTATTAT64300.015.6697551
ATTATAC61150.015.5379453
GTATATA123600.015.4192481
TAGACTG30000.015.360595
TATACAG48900.015.1556025
ACTGTGC39600.014.8736918
Produced by FastQC (version 0.11.2)