FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l08n01_182.3510000006b22e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l08n01_182.3510000006b22e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9317314
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG57000.024.0915831
CTATACT75350.020.1708834
CCTATAC59950.019.9652233
TATACTG53400.018.4987755
TATAGGG55350.018.4501532
TAGACTG29700.018.3891775
TATACTA97900.017.9489865
CCCTATA58450.016.577722
TTATACT67900.016.3683264
TAATACT65650.016.2781854
GTATTAG39800.015.1622271
GTATTAT62600.015.0291091
TATACAG52500.014.9260725
TATACCG25800.014.7262165
GTATATA118950.014.7002831
GTATTAC42300.014.3784461
GTATTGG59200.014.3672511
TAGGGAT62200.014.2793814
TAGACAG30400.014.0601445
TACACTG43850.013.9714355