FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l08n01_176.3510000006b1dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l08n01_176.3510000006b1dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18112949
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG115500.018.1742045
CTATACT112850.017.0872864
TAGACTG58850.016.5433125
TACACTG84850.015.2800885
ACTGTGC79450.015.1829058
TAATACT136150.015.1747054
GTATAGG78450.015.1432391
TATACAG100950.014.9131035
CCTATAC73850.014.7920913
GTATTAG82600.014.7851181
GTATTGG155500.014.7600721
GTATTAT127300.014.6701941
GTATATA243700.014.6243791
TTATACT136950.014.5311734
TATACCG56850.014.4520915
TCTATAC90550.014.4243573
ATTATAC122150.014.2700283
ATAATAC124750.013.9726183
GTGTAGG78600.013.9656511
GTCTTAG53550.013.931951