FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l08n01_174.3510000006b0dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l08n01_174.3510000006b0dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15620278
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG104250.019.9080375
CTATACT108800.017.635854
TAATACT136900.017.1382874
TATACAG97050.016.8339485
GTATTAT126550.016.4082431
TAGACTG55200.016.0888735
TATACCG51450.015.8769155
TTATACT134800.015.6436124
ATAATAC121300.015.5836423
GTATAGA89350.015.3158371
GTATTAG81050.015.2427421
GTATAAG72650.015.2392321
GTATTGG130150.015.0416621
ATACTGT109000.015.0319626
TACACTG76900.014.88385
GTATATA233150.014.8775081
GTATAGG70600.014.8740881
ACTATAC86250.014.8128943
CCTATAC71250.014.7317433
TCTATAC85800.014.6691633