FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l08n01_164.3510000006afc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l08n01_164.3510000006afc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8332946
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG50950.021.07851
CTATACT67800.020.5943474
TATACTG51800.020.5364425
TATAGGG48500.018.6064322
CCTATAC54650.018.1631743
TATACAG50950.017.523495
TAATACT73050.017.1638264
TTATACT69150.016.964274
TATACCG25400.016.827355
GTATTAT63000.016.7451041
GTATAAG41450.016.6233631
TATACTA96100.016.1102095
ATTATAC60350.015.89683
GTATATA125500.015.6001561
TCTATAC44250.015.5628933
GTATTAG41950.015.4057341
TACACAG34850.015.3986485
TACACTG38750.015.3195235
ATAATAC61650.015.2534383
ACTATAC43950.015.128813