FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l07n02_140.3520000006b238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l07n02_140.3520000006b238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731221
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC20280.11714275647072211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC7700.023.4420728
GTATAGG9300.022.488551
TAGGACT7400.020.5451434
TAATACT17300.020.3224724
GTATTGG12250.020.177111
GTCTAGG4850.019.6010821
GTATAGA10950.019.5339531
TTATACT17250.019.2796824
ATACTGT12000.019.0004166
TACACAG7300.018.8713675
GTCCTGG5850.018.6880361
AATACTG9500.018.5014725
TATACTG12650.018.4006755
TACACCG5300.017.9259575
CTAGACT6900.017.9025634
GTGTAAG5600.017.8247321
ACTGTGT9950.017.6637028
GTCTTAC4650.017.3775161
CTACACT9300.017.3694844
TACTGTG8550.017.2225997
Produced by FastQC (version 0.11.2)