FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l07n02_139.3520000006b1a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l07n02_139.3520000006b1a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33280093
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG221350.021.0346535
CTATACT294350.019.2895774
TACACTG164000.018.540655
TAGACTG113400.018.4343745
CAGAGGT215200.018.1708984
TTATACT347750.017.7074784
TTACACT199750.017.6258564
GAGGTGT219550.017.373926
GTATTGG251350.017.1917171
TAATACT362050.017.047454
CTACACT161400.017.0448784
CAGGACT104900.017.0306474
GTATAGG197700.017.023971
ACTGTGC159800.016.943568
ATACTGT239950.016.9064666
CTGTACT192500.016.8087434
CTGTGCG76350.016.7973619
TAAGACT162100.016.6781584
TAAGGCT144350.016.6553044
CTACCCT130200.016.604254