FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l07n02_135.3520000006b12c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l07n02_135.3520000006b12c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15943135
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG99100.020.1355695
CTATACT113850.018.9470864
GTATAGG85750.018.9045981
ATAATAC111200.017.5186253
GTATTAT113800.017.5032621
TAATACT124300.017.277744
GTATATA220100.017.0413571
CCTATAC87050.016.6476153
ATTATAC114550.016.6329883
GTATTAG79600.016.30411
ACTGTGC77550.016.2317948
TATACCG60550.016.1637215
TTATACT131400.015.693294
GTATAAT123650.015.6860391
ATACTGT98650.015.408666
TAGACTG54850.015.2448915
GTATAAG75950.015.0846941
TCTATAC83900.014.9507493
TACACTG78500.014.82807355
GTATTGG117650.014.7889171
Produced by FastQC (version 0.11.2)