FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l07n02_133.3520000006b0cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l07n02_133.3520000006b0cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37326684
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG257950.020.4445255
CTATACT313600.018.6660884
TTATACT411850.017.5587774
TAATACT423250.017.5348784
TAGACTG123900.017.1022455
TACACTG175200.017.0842555
GTATAGG211700.017.0656681
GTATTAC195150.016.9296041
ACTGTGT200450.016.7303058
ACTGTGC173950.016.6029158
CTGTGCG83550.016.5436089
ATACTGT276300.016.401446
TTACACT235650.016.3520454
CTACACT184300.016.1386664
GACTGTG108200.015.6729117
CTAGACT147450.015.56399254
TACTGTG194300.015.4019767
GGACCGT75550.015.0900726
TCTATAC250750.015.06419853
TAGGACT150300.015.0159674