FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l07n02_132.3520000006b060.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l07n02_132.3520000006b060.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23647365
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG155850.023.639561
CTATACT199950.020.9351334
CCTATAC160950.020.8123973
TATAGGG149050.018.6512722
TATACTG146150.018.3671365
CCCTATA149850.017.7906062
TATACTA267900.017.592635
TATACCG76800.017.4453535
GTATATA328800.016.713621
GTATTAT170250.016.3906231
TCTATAC122300.016.084143
TTATACT190950.016.0494924
TAGACTG84900.016.0047975
ACTGTGC110000.015.5459798
TAATACT183500.015.5358984
TATACAG146200.015.4036215
GTATTAG107500.015.3007291
GTATAAT173950.015.1128091
ATACTGT155750.015.03626
ATTATAC168150.014.8067033