FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l07n02_131.3520000006b02d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l07n02_131.3520000006b02d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5749484
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGGT42800.027.4196454
CTGACCT23800.025.3533574
GAGGTGT42300.024.8183966
AGAGGTG53600.023.1347895
TCAGAGG33900.022.8452853
ACTGCGC12850.021.4408958
TATACTG35700.021.2932325
TAGAGGT28500.021.0055664
CTGTACT35800.020.9692424
TAGACCT25900.020.9128764
TACTGCG12600.020.7352927
CCTCTGA37900.020.6957041
ACTGTGC29850.020.687938
TCAGAAC31350.020.611523
CTAGGCT22900.020.5403754
GAACCGT13700.020.4575316
CTGTGCG16800.020.3570469
AGGTGTA39650.020.247047
TGACCTG26300.020.2326185
TAACCTT39550.020.181465
Produced by FastQC (version 0.11.2)