FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l07n01_140.3510000006b23b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l07n01_140.3510000006b23b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731221
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC19250.11119319832649906No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG18410.10634113149043363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT3950.024.047516
TATACTG10750.022.531965
GTCTTAG5300.021.5153431
TTATACT16650.021.1095894
CTATACT14500.019.9813184
GTATAGG10100.019.2874741
ACTGTCC4450.019.2109898
TGGACCG8850.018.7828715
GTATAAT13750.018.6596681
CTACACT9300.018.3857734
TATACCG5500.018.134015
TACTGTG9450.018.0928887
TACACTG7450.017.8500335
GTATTGG11600.017.6125931
ACTGTGC8400.017.5274878
ACACTGT9250.017.4571936
TAATACT17550.017.0500554
TATCCTG7800.017.0490685
ATAATAC13950.017.0238633
TCTATAC10350.016.9794523
Produced by FastQC (version 0.11.2)