FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l07n01_139.3510000006b1a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l07n01_139.3510000006b1a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33280093
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG219800.021.13225
CTATACT299100.019.262094
TACACTG167000.019.139575
TAGACTG112350.018.5154635
TTATACT345200.017.529024
TAACACT188350.017.4501634
ACTGTGC155500.017.4396328
CTGTGCG69750.017.3631139
CTACCCT130200.017.145334
ATACTGT233050.017.1183476
GTATAGG203300.017.0406721
CTACACT161200.016.9418894
CTAGACT136600.016.8983614
TTACACT206700.016.8660964
GTATTGG251200.016.8370881
GAGGTGT228700.016.7378836
CAGAGGT212950.016.683344
TTAACCT199750.016.3829234
TAATACT362100.016.3567474
TTACCCT149750.016.0488054