FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l07n01_135.3510000006b12f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l07n01_135.3510000006b12f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15943135
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG107450.020.11135
CTATACT120250.019.1566874
GTATAGG86350.018.8220671
CCTATAC87550.017.5235063
TTATACT127950.016.8902024
TAATACT124750.016.6381344
TATACCG60300.016.4612985
GTATATA217200.016.3661041
GTATTAT120150.015.9003181
TATACAG99600.015.8312025
ATACTGT108300.015.2610546
GTATAAT116700.015.1487081
ATAATAC114750.015.0254873
TATACTA160150.015.0057855
GTATAGA83650.014.99828151
TCTATAC82650.014.8844073
ATTATAC113150.014.7342213
GTATTAG77350.014.683891
TAGACTG55550.014.6199945
GTATAAG75350.014.4429451