FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l07n01_131.3510000006b020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l07n01_131.3510000006b020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5749484
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGGT42500.030.62174
TCTGGCC27450.026.3014833
GAGGTGT43450.025.5778186
AGAGGTG51600.025.4959555
GCTCAGA22000.024.1911031
TCAGAGG32900.023.965763
CCTCTGA36950.023.4054641
GACTGTG18250.022.6409057
TTACCCT26500.022.2250864
TAGACCT24100.021.8762864
AGGTGTA36200.021.6477537
TTAACCT35550.021.2434544
TTACACT34350.020.8793934
CAGACCT19150.020.8342634
CAGGACT19250.020.7260324
TAGGGCT25450.020.7158534
CTGACCT23650.020.6858654
CTGTGCG14750.020.6075049
TAGACTG20300.020.5886485
TGTACTG44750.020.483565