FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l06n02_109.3520000006b11f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l06n02_109.3520000006b11f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40322377
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG281900.019.067945
TTATACT396750.017.358214
TAATACT424600.017.059194
CTATACT297000.016.9470064
TATACAG249900.016.07045
ACTGTGC177300.015.8861338
TAGACTG129700.015.72003655
TACACTG190750.015.123785
TAGACAG134150.014.9860065
TCTATAC256300.014.9284893
GTATAGG188950.014.721541
CTACACT191300.014.6585324
CTAGACT152150.014.55684954
ATACTGT301100.014.5135116
GTATAAT365550.014.4254391
TATACCG125700.014.405425
GTATAGA264600.014.3762111
ATTATAC356950.014.2876533
GTATTAT377200.014.2824431
TAGGACT169650.014.2038994