FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l06n02_104.3520000006b0a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l06n02_104.3520000006b0a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4387812
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG30600.020.346885
TAATACT44250.018.5829854
ACTGTCC13750.017.6174558
TTATACT44750.017.3131944
TACACTG22450.017.1481255
CCTACAC18200.016.9759653
CTATACT31950.016.8108734
GTATTAC23000.016.746081
TAGTACT48650.016.60924
TACCCTG16700.016.506675
GTAATAC23600.016.3141923
GTATAAT40550.016.2997131
CTACACT23800.016.176734
TATACAG27200.016.075595
GTATAAG21300.016.0734421
ATACTGT30200.015.8856516
TAGACAG15600.015.842615
ATAATAC38250.015.7821023
AGTACTG26900.015.7248225
GTATAGA27050.015.6451161