FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l06n01_118.3510000006b32d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l06n01_118.3510000006b32d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19235300
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG122500.018.6115425
CTATACT130400.017.6300684
TAATACT153850.016.9805364
TATACAG115600.016.7229825
CCTATAC97500.016.6616443
TTATACT153450.016.6223964
TAGACTG64750.016.5785245
ACTGTGC87100.016.2506088
GTATAGG94500.016.2432371
GTATTAT138050.016.145011
GTATATA271750.015.7738931
ATTATAC139650.015.74843
GTATAGA103850.015.5587361
GTAATAC90000.015.094543
GTATAAT143000.014.6556281
TACACTG90950.014.6228665
TCTATAC97800.014.6192133
ATAATAC140500.014.5374593
TATACCG64200.014.1310855
GTATTAG92150.014.1304861