FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l06n01_116.3510000006b28b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l06n01_116.3510000006b28b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18305124
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG117100.017.1982695
CTATACT122050.016.8516544
GTATAGG91100.015.2841821
TTATACT143850.015.2554314
TATACAG106800.014.8097865
GTATAAG85100.014.6865281
CCTATAC89750.014.6074813
TAATACT151250.014.32062154
GTATATA263350.014.2556411
TACACTG81400.014.2377455
TAGACTG61350.014.1681235
GTATTAT139550.014.1322461
TATACCG58350.014.0011415
ACTGTGC80400.013.646688
GTATTAC87050.013.5932571
ATTATAC135250.013.591723
GTATAGA98900.012.9255471
TTACACT90800.012.921224
TATACTA174950.012.7602645
ATAATAC136450.012.7411393