FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n02_80.3520000006b450.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n02_80.3520000006b450.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13841199
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG97700.022.0866175
TAGACTG45450.020.0786825
CTATACT127650.019.6602424
ATACTGT99850.019.2214496
TTATACT171000.019.2069174
TAAGGCT60850.018.8248794
TACACTG65150.018.7493655
CTACACT64250.018.49454
TAATACT184950.018.1180114
GTATAGG82000.017.168221
ACTGTGT79350.017.0605358
AATACTG85600.017.0463925
TTACACT95500.017.0215234
TAGGACT57050.016.9961384
GTATTAC82900.016.9818341
TATCCTG63650.016.9515
ACTGTGC68300.016.8997528
TCTATAC101300.016.7045653
TAGACAG45700.016.53675
CTGTACT87100.016.5348474