FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n02_79.3520000006b3ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n02_79.3520000006b3ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32389569
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG191650.020.6221081
CTATACT232000.019.442194
CCTATAC196350.019.0516953
TATACTG177200.016.8444525
TATAGGG182150.016.2609022
TATACTA302950.015.4377895
CCCTATA183300.015.0954572
ACTGTGC147800.014.6226568
TATACCG102900.014.4573915
GTATATA384850.014.3353181
TATACAG180100.014.3036385
TAGACTG107250.014.2698555
TAGACAG104550.014.0928425
GTATTGG190800.013.9588781
TAATACT220950.013.9180154
TACACTG148400.013.8360085
TCTATAC151500.013.6792943
TACACAG135000.013.6602585
GTCCTAG116000.013.4889691
GTATAAG142000.013.4641381