FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n02_78.3520000006b351.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n02_78.3520000006b351.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15480110
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG100650.020.069035
TAATACT124850.017.5117264
ATAATAC111900.017.1181053
GTATTAT112450.016.6637651
CTATACT93600.016.6554994
TTATACT121050.016.647954
GTATAAT113000.016.1196881
TAGACTG50450.016.0154445
ATTATAC106650.015.8660893
TACACTG75150.015.6213735
ACTGTGC72800.015.26759058
GTATATA219500.015.1886721
TATACAG90300.015.1584915
GTATTAG75250.015.1052431
GTATAAG72550.015.077411
GTATAGA83750.015.0486441
TCTATAC77200.015.0228823
TATACCG49650.014.9333295
CCTATAC67300.014.760873
TAGACAG53550.014.644545