FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n02_76.3520000006b2d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n02_76.3520000006b2d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13417446
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC270620.20169263211493454No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG231080.1722235364315981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG101500.022.4304355
TACACTG68250.020.474485
CTATACT112800.019.4252454
TAATACT170450.019.2967324
TTATACT160200.019.2259224
ATACTGT96200.018.9630156
TAGACTG43000.018.6804125
GTATTAG89400.016.8642481
GTATAGA106350.016.8596731
TTACACT89400.016.7474124
CTAGACT51950.016.6517734
AGTACTG80150.016.604385
ACTGTGC60450.016.5797928
AGACTGT45950.016.541916
GTATTGG130000.016.5363771
GACTGTG36950.016.456427
TAAGACT71450.016.4312044
TAAGGCT54450.016.4109864
ACTGTCC43850.016.3568738
TATACAG86250.016.2015465
Produced by FastQC (version 0.11.2)