FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n02_75.3520000006b252.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n02_75.3520000006b252.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17298131
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT138100.021.8549254
GTATAGG108600.021.5472131
TATACTG107500.020.9127865
TAATACT140600.020.0465034
CCTATAC118650.019.3895723
TTATACT145250.018.6193874
TATAGGG103950.018.386072
TATACTA184050.017.6894365
GTATTAT131350.017.6703661
TAGACTG59200.017.2613965
GTATATA237550.017.2186551
ACTGTGC82950.016.949658
ATAATAC124500.016.4550253
ATTATAC129000.016.3600223
TAAGACT65400.016.2794424
ATACTGT111250.016.2693656
GTATTAG83600.016.2141571
CCCTATA104800.016.1501312
CTAATAC82850.016.0640893
GTATAGA93450.015.77751