FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n02_72.3520000006b1bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n02_72.3520000006b1bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5594298
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG35050.021.4263445
TAGACTG20050.020.1503945
GTATAGG35100.020.0543941
TTATACT48250.019.5055664
CTATACT48500.019.3070144
TAATACT47500.018.2124544
TATACTA67950.017.7673745
CCTATAC37600.017.5727983
GTATAGA29000.017.5485291
TATACAG33850.017.4117345
ATACTGT38400.017.3202616
GTATTAT41850.017.0471081
ACTGTGT30800.016.964518
TCTATAC27350.016.6850643
GTATAAT44500.016.6732221
GTATTAG28450.016.5503771
ATAATAC42350.016.3875273
GTATATA80750.016.256291
TATACCG19650.016.2065755
GTATTAC29850.016.0928131