FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n02_69.3520000006b139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n02_69.3520000006b139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30118769
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG211300.025.073941
CTATACT273250.022.6818964
TATACTG201900.021.821965
CCTATAC219800.020.6303753
TTATACT258650.020.1767754
TAGACTG109950.020.057335
TATAGGG200350.019.8383312
TAATACT262000.019.4471244
GTATTAT242250.019.1808431
TATACAG189600.017.8731695
TATACTA356300.017.8080275
ACTGTGC154850.017.392588
ATTATAC235900.017.1066973
TATACCG99150.017.0650545
GTATTAC152150.016.9732421
ATAATAC232600.016.8589573
GTATAGA173600.016.793751
CCCTATA208250.016.6429652
ATACTGT217600.016.417846
GTATAAT245950.016.3011281