FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n01_80.3510000006b453.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n01_80.3510000006b453.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13841199
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG98900.020.7477365
TTATACT168500.018.8031734
TACACTG67500.018.6477055
TAAGGCT57200.018.1036324
CTATACT127650.018.085094
TAGACTG48250.017.9166725
GTATAGG80800.017.764391
TAATACT179950.017.6331484
AGTACTG79250.017.261425
CTAGACT56950.017.015384
GTATAGA111400.016.8952561
GGACTGT40850.016.5111776
ACTGTGC65550.016.4488268
CTGTACT78600.016.1963634
AGGACTG45750.016.1963355
TGTACTG106600.016.1745685
TAGACAG45300.016.1475185
ATACTGT102550.016.0722066
TTACACT93900.016.0361064
CTGTCCG26400.016.012829