FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n01_79.3510000006b3cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n01_79.3510000006b3cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32389569
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG191450.021.4234541
CTATACT239600.019.7458534
CCTATAC196600.019.016453
TATAGGG177650.016.6062432
TATACTG184450.016.5325685
TATACTA313050.015.6888385
CCCTATA189750.015.2969332
TAATACT225100.015.1935894
TACACTG145800.014.627595
TATACAG187800.014.5936595
GTATATA403850.014.5927021
CTACACT148550.014.54934
TATACCG101700.014.5252425
GTATTAT207300.014.4435961
ACTGTGC148700.014.3422958
GTATTGG189900.014.3404831
TAGACTG107800.014.276125
TACACCG115350.014.0417335
TCTATAC147800.013.9171953
TTATACT227950.013.8575174