FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n01_76.3510000006b2db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n01_76.3510000006b2db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13417446
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC271990.20271369081716448No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG230910.17209683571672282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG102250.023.1339595
TACACTG70500.019.8080675
TAGACTG44350.019.4922375
CTATACT111850.018.8986424
TAATACT174700.018.326254
ATACTGT101700.018.168166
CTAGACT52000.017.995734
TTATACT162550.017.971934
AGACTGT45550.017.6230326
CCAGGAC37500.017.6084383
TAGACAG44700.017.320645
GACTGTG39350.017.2613057
TAAGGCT53450.017.0631854
TATACCG43350.016.8739015
TATACAG85200.016.836485
TAGGACT56050.016.780164
TTACACT93550.016.5023634
ACTGTGT69200.016.4049198
ACTGCGC22750.016.2852928
ACTGTGC60600.016.1464448
Produced by FastQC (version 0.11.2)