FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l05n01_72.3510000006b1b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l05n01_72.3510000006b1b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5594298
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT49650.021.6221144
GTATAGG37000.020.5550481
TTATACT46300.020.5190854
TATACTG35800.020.0343725
GTATTAT43600.019.623941
TAATACT48700.019.117724
CCTATAC38200.017.7834743
CTAATAC29450.017.5826823
ATTATAC41750.017.0678463
AATACTG33350.016.9485655
TAGACTG19900.016.9467755
CCCTATA36750.016.8049682
GTATAAA52800.016.7447721
TACACTG27850.016.5435355
TATACTA66150.016.2996315
TATACTC39400.016.2749375
TCTATAC29800.016.2602733
TATACCG17000.016.205445
ATACTGT40550.016.0476536
ACTATAC34100.016.0209183
Produced by FastQC (version 0.11.2)