FastQCFastQC Report
Tue 25 Oct 2016
C98UFACXX_l04n02_162.3520000006b300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98UFACXX_l04n02_162.3520000006b300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20098136
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG126800.024.9736961
CTATACT159050.022.051194
CCTATAC136150.021.8170223
TATAGGG120900.019.3839572
TATACTG118450.019.0170865
CCCTATA128650.017.58812
TAGACTG72500.017.30485
TATACTA214550.016.701045
TATACCG66900.016.1960625
TAGGGAT130400.016.108464
GTATTGG121150.015.8164641
TACACCG69650.015.6930515
ACTGTGC95350.015.4441658
GTGTAGG85900.015.1664941
TACACTG94050.015.1082135
CTACACT92800.014.9535474
ATAGGGA130850.014.8916653
GTATAGA100050.014.7323631
GTATATA258750.014.5352971
TATACAG116150.014.4838735